Comparative Study of Antioxidant in Radish (Raphanus Sativus L.) At Different Areas of District Khairpur Mir's
The sample of three varieties of Radish (TakeeJapane, Neutech, F-1 Hybrid) was collected during the winter season from various parts of the district of Khairpur Mir's. The physical properties of each sample, like moisture content and PH etc., were determined immediately after collection within 24 hours. Extraction was done using three types of solvents i-e methanol, water, and methanol: water (1:1) separately. Using a double beam spectrophotometer, each Variety's free radical scavenging efficacy was evaluated in 2, 2-diphenyl-1-picrylhydrazyl (DPPH). After that, half-maximal inhibitory concentration IC50 of each property was calculated. The IC50 value of each Variety was found very close to (58.92) µg/ml standard ascorbic acid indicating that Radish is a good antioxidant. The observed IC50 values of the three varieties of the Radish was found in the range of 59.688 to 66.088 µg/ml (TakeeJapane), 44.229 to 68.347 µg/ml ( Neutech) and 31.644 to 57.65 µg/ml (F-1 hybrid). The data indicate that IC50 values F-1 hybrid Variety were lowest than other two varieties indicating better antioxidant.