Comparison Of The Effectiveness of rbcL and matK in Amplifying The Genome of Diospyros
Ebenaceae is a large angiosperm family that includes many endemic species; one of the genera is Diospyros. The aim of identified chloroplast genome marker in the Diospyros family is to enable precise species identification by analyzing a unique DNA sequence of a target gene. The current study was carried out to create a marker for different species of Diospyros growing in South Sulawesi. Three putative species of Diospyros (each represented by specimens collected from two districts in South Sulawesi) were evaluated using two regions in the chloroplast genome (matK and rbcL) in order to discriminate them at the species level. Results showed that matK yielded 891 bp after alignment. However, there was no precise identification to species level. The combined rbcL gene showed 100 percent amplification in three species, but combined MatK gene just 50 percent showed amplification. Considering the overall performance of these loci, we suggest the rbcL gene for amplification and matK + rbcL combination may amplify to determine Diospyros growing in South Sulawesi to the species level as distinguished on morphological grounds. These findings show the necessity of finding other candidate genes or markers that can potentially help delineate the various species of Diospyros and other related Diospyros genera. This study is very needed, especially for endemic species.