INDIVIDUAL AND COMBINED HORMONAL EFFECTS ON POTATO TOWARDS IN-VITRO ELIMINATION OF LEAF ROLL VIRUS

Abstract

The experiment was performed to study the response of four different growth regulators in special combinations and levels on potato for the elimination of potato leaf roll virus and mass propagation of potato cultivar SH5. Growth regulators IAA, Gibberellic acid, Kinetin and Pantothenic acid were applied in various combinations and concentrations. Nodal cuttings of SH5 were exposed to MS media supplemented with four different treatments of Kinetin and Giberellic acid starting from 0.02 to 1.00 mgL^-1 individually. Combination of kinetin and IAA, Pantothenic acid and Gibberellic acid were used in MS media in which level of one hormone was kept constant while other was varied. Maximum number of leaves, nodes and highest shoot length was obtained at 0.04 mgL^-1 kinetin + 1.00 mgL^-1 of IAA. ELISA test was performed to screen the potato leaf roll virus free potato plants and the results showed that 80% in-vitro developed plants were infection free.