Microbiological Analysis of Drinking Water of Gravity Flow Water Scheme Abbottabad, Pakistan
Abstract
Since pure drinking water is good for health, it must be assessed for bacterial contamination. This research was conducted on the gravity flow water scheme of District Abbottabad, Pakistan. Pakistan. Twenty water samples were collected in different areas of city. Physiochemical analyses including pH, temperature, and chlorine residue showed that all water samples were according to prescribed values by the World Health Organization. This research work aims to analyze the total viable count (TVC), Total Coliform Bacteria (TCB), Total fecal Coliform Bacteria (TFCB) present abundantly in drinking water. The results showed that highest TVC values 5.6020 log cfu /100ml and lowest were 3.7781 log cfu/100ml, TCB were found in the range 250>2.0MPN/100ml, TFCB were found in the range 25>1.1MPN and E.coli were found in all water samples except sample-11. The water samples analysis data indicated that 100% water samples were unfit due to high TVC values, 85% samples were unfit due to higher (TCB) and 45% TFCB than permissible limits and 75 % samples were found to be contaminated with E. coli. Escherichia coli, Klebsiella oxytoca and Pseudononas aeruginosa were identified based on morphology, gram staining and conventional biochemical tests and the identification was further verified by API 20 E kit.