The Effect Of Different Essential Oil Extraction Methods On The Efficiency And Antibacterial Properties Of Myrtus Communis L. Leaves
The effect of different essential oil extraction methods on the efficiency, composition and antibacterial properties of M. communis L. leaf essential oil was studied. M. communis L. essential oil was prepared by three methods: hydro distillation, steam distillation, and hydro-steam distillation. The highest essential oil efficiency was related to hydro-steam distillation, which was significantly different from the other two methods. 29 essential components were identified in GC/MS analysis, of which 7 essential components, Î±-pinene, 1,8 -cineol, linalool, decane, linalyl acetate, Î±-terpineol, camphene, were the most predominant compounds and showed significant differences in the three different essential oil extraction methods. The antibacterial effect of essential oils obtained from the three distillation methods in different concentrations on three Gram-positive bacteria) Staphylococcus aureus, Streptococcus mutants, Staphylococcus epidermidis) and three Gram-negative bacteria) E. coli, Pseudomonas aeruginosa, Salmonella typhimuriu (was investigated, which revealed significant differents among them. The important result was that the essential oil obtained by steam distillation at a concentration of 80Î¼L/mL had a more active antibacterial effect on Staphylococcus aureus and Streptococcus mutans, to the extent that it had the same effect as antibiotics, and in some cases, it was even more active than antibiotics.