EFFECT OF SUCROSE ON IN VITRO CALLOGENESIS, EMBRYOGENESIS AND ORGANOGENESIS OF Dendrobium sabin H.

Abstract

Protocorms of dendroboum sabin H were proliferated on Murashige and Skoog (MS) medium supplemented with 0%,1,5%,3%,5%,7%,9%,11% and 13% sucrose to determine the callus growth , embryogenesis and organogenesis  Maximum weight of callus was found to be 365 mg after 20, 40 and 60 days of culture on the medium supplemented with 5% sucrose level. The results showed that there was continuous increase in fresh weight of callus at 20, 40 and 60 days after inoculation.  Maximum callus growth was observed when the sucrose concentration was 5%. Lowest and highest levels of sucrose i.e. 0% and 13% showed no growth. The 5% level of sucrose was found to be the most appropriate for the induction of callus timely and efficiently as well. It was also found that 5% level of sucrose took significantly less days (20 days) to form somatic embryoids. Maximum embryogenesis percentage (100%) was observed at 5% followed by 3% (77.77%) sucrose level. No embryogenesis took place at 0.0% and 13% levels of sucrose. The 1.5%, 9% and 11% resulted in a very poor embryogenesis. The 5% level of sucrose produced significantly more number of shoots. Length of the developed shoots was also significantly high at this level than any other level.