Molecular Spectroscopic Study on Interaction between Eugenol and Hesperidin Nanoparticles
Parkinson’s disease is characterised by a progressive loss of dopaminergic neuron in the substantia nigra. Hesperidin is a free radical scavenger that has been shown to have a protective effect against oxidative insults. An antioxidant can neutralise the free radical by accepting or donating an electron to eliminate the unpaired condition. Ionic gelation with tripolyphosphate and chitosan was used to render hesperidin-loaded chitosan nanosuspension. UV-Visible, FTIR, SEM, XRD, and a particle size analyser were used to classify hesperidin nanoparticles. Eugenol association with hesperidin nanoparticles was investigated using spectroscopic methods such as UV-Visible, Fluorescence, Synchronous fluorescence, lifetime measurements, FRET and antibacterial activity. Eugenol–Hesperidin nanoparticles were found to have a complex formation. The distance between the donor and the acceptor was determined using Forster's non-radiation energy transfer principle. Antibacterial behaviour was investigated as well.